Journal: Journal of vascular research

Article Title: A Novel Model of Balloon Angioplasty Injury in Rat Arteriovenous Fistula

doi: 10.1159/000507080

Figure Lengend Snippet: (A) α-SMA and CD68 immunostaining of representative sections from Group 1 and Group 2. Scale bar=100 μM. Higher magnification images are at 40X. (B) Quantification of α-SMA (intimal region) and CD68 (intimal and medial regions) expression from AVF-artery and AVF-vein. N=3–4 in each group. *p<0.05, **p<0.001. An unpaired t-test was used to test for statistical differences between AVF without angioplasty and AVF with angioplasty groups.

Article Snippet: Immunohistochemical Analysis Paraffin embedded arterial and venous sections were evaluated to identify the cells contributing to the vascular remodeling following balloon angioplasty. α-SMA (14-9760-82, Invitrogen) and CD68 (NB600–985, Novus Biologicals) primary antibodies were used to verify the presence of smooth muscle cell phenotype and inflammatory cells respectively. α-SMA expression in the intimal region and CD68 expression in both medial and intimal regions of AVF vessels were quantified using Cellsense Dimension Software (Olympus Life Science) on digital photographs taken at a final magnification of 20X.

Techniques: Immunostaining, Expressing

Journal: iScience

Article Title: A rat liver cell atlas reveals intrahepatic myeloid heterogeneity

doi: 10.1016/j.isci.2023.108213

Figure Lengend Snippet:

Article Snippet: Mouse anti-rat CD68 AF700; Clone: ED1 , Novus Biologicals , Cat# NB600-985AF700.

Techniques: Recombinant, SYBR Green Assay, Staining, Single Cell, Selection, Gene Expression, Software

Journal: Communications Biology

Article Title: MicroRNA-221-3p inhibits the inflammatory response of keratinocytes by regulating the DYRK1A/STAT3 signaling pathway to promote wound healing in diabetes

doi: 10.1038/s42003-024-05986-0

Figure Lengend Snippet: Representative immunohistochemistry images ( a , b ) and summary data ( c , b ) showing myeloperoxidase (MPO) ( a , c ) and CD68 ( b , d ) expression levels on days 1, 3, 7, and 11 after wound formation in diabetic mice injected with miRNA mimic negative control (DM-Ctrl) or miR-221-3p agomir (DM-miR-221-3p). Density of positive cells was calculated using the following formula: number of positive cells/area size ( n = 4–6). Scale bars, 50 μm. Summary data showing relative mRNA expression levels of IL-1β, IL-6, IL-8 , and TNF-α at the edge of the skin wound on days 1 ( e ), 7 ( f ) and 11 ( g ) ( n = 6–10). h Summary data showing relative mRNA expression levels of CD86 and CD206 at the edge of the skin wound on days 11 ( n = 6–10). The mRNA levels were normalized to 18S ribosomal RNA. Data in c-h are presented as mean ± SEM. Solid circles represent DM-Ctrl group and squares, DM-miR-221-3p group. * P < 0.05, ** P < 0.01 for DM-miR-221-3p vs. DM-Ctrl.

Article Snippet: Sections were then incubated overnight at 4 °C with primary antibody CD68 (NB600-985, Novus Biologicals, USA), MPO (AF3667, Abcam, UK) or DYRK1A (DF3270, Affinity Biosciences, China) followed by incubation with a secondary antibody conjugated with streptavidin-horseradish peroxidase.

Techniques: Immunohistochemistry, Expressing, Injection, Negative Control

Journal: Communications Biology

Article Title: MicroRNA-221-3p inhibits the inflammatory response of keratinocytes by regulating the DYRK1A/STAT3 signaling pathway to promote wound healing in diabetes

doi: 10.1038/s42003-024-05986-0

Figure Lengend Snippet: Representative immunohistochemistry images and summary data showing myeloperoxidase (MPO) ( a ), CD68 ( b ) and DYRK1A ( c ) expression levels in skin wound tissues of wild type (WT) and Mir221 knockout (KO) mice. Data are presented as mean ± SEM ( n = 5–8). Scale bars, 50 μm in ( a , b ), 5 μm in ( c ). d Representative images and summary data showing expression levels of phosphorylated (p)-STAT3 (Tyr705), p-STAT3 (Ser727), total STAT3, and DYRK1A in skin wound tissues of WT and Mir221 KO mice. Vertically stacked strips of bands in a figure are not derived from the same membrane in any case. Data are presented as mean ± SEM ( n = 5), * P < 0.05.

Article Snippet: Sections were then incubated overnight at 4 °C with primary antibody CD68 (NB600-985, Novus Biologicals, USA), MPO (AF3667, Abcam, UK) or DYRK1A (DF3270, Affinity Biosciences, China) followed by incubation with a secondary antibody conjugated with streptavidin-horseradish peroxidase.

Techniques: Immunohistochemistry, Expressing, Knock-Out, Derivative Assay, Membrane