Journal: Stem cell research & therapy

Article Title: Pluripotent stem cell-induced skeletal muscle progenitor cells with givinostat promote myoangiogenesis and restore dystrophin in injured Duchenne dystrophic muscle.

doi: 10.1186/s13287-021-02174-3

Figure Lengend Snippet: Fig. 4 Givi-MPC decreased inflammation and muscle necrosis in Mdx/SCID mice 7 days after CTX injury. a Representative images of HE and Masson's trichrome staining in Mdx/SCID mice after human myoblasts or control MPC or Givi-MPC transplantation 7 days after CTX injury. Black arrows point at infiltrated inflammatory cells. b, c Quantification of TA muscle fiber necrosis after treatment with MPC 7 days after CTX injury. Human myoblasts: n = 3; control-MPC: n = 4; Givi-MPC: n = 5. d Quantification of CD68-positive cells in TA muscle following MPC transplantation 7 days after CTX injury. n = 4. e Representative images of macrophages (red, CD68) and human cells in TA CTX-injured muscle of Mdx/SCID mice following MPC transplantation. The boxed area is shown below at high magnification. Bar = 100 μm

Article Snippet: The muscle sections were incubated with primary antibodies including Laminin (L9393, Sigma, 1:500 and L0663, Sigma, 1:300), dystrophin (D8168, Sigma, 1:200), human-specific dystrophin (NBP2-79783, Novus, 1:200), green fluorescent protein (GFP, #2956, Cell Signal Technologies, 1:500), dystrophin (ab15277, Abcam, 1:200), human nuclear antigen (NBP2-34342, Novus, 1:100), CD68 (NB600-985, Novus, 1:200), CD31 (NB600-562, Novus, 1:200), Pax7 (ab187339, Abcam, 1:300), and human LaminA/C (NBP2-59933, Novus, 1:200) at 4 °C overnight respectively and anti-rabbit/mouse/rat secondary antibodies conjugated to Alexa Fluor 594 or Alexa Fluor 647 or Alexa Fluor 488 (Life Technologies) at room temperature for 1 h. Images were taken using a confocal microscope (FV1000, Olympus, Japan).

Techniques: Staining, Control, Transplantation Assay

Journal: PLoS ONE

Article Title: Silver and Gold Nanoparticles Exposure to In Vitro Cultured Retina – Studies on Nanoparticle Internalization, Apoptosis, Oxidative Stress, Glial- and Microglial Activity

doi: 10.1371/journal.pone.0105359

Figure Lengend Snippet: Fluorescent immunostaining: Iba1 (green) and ED1 (red) for detecting microglial cells (DAPI, blue). In the NP-exposed retinas (20 nm Ag (G–I), 80 nm Ag (J–L), 20 nm Au (M–O), 80 nm Au (P–R)) as well as in the control retinas (A–C) the Iba1- and ED–positive cells were located in the IPL, INL and GCL in all groups (A–C). AgNO 3 -exposed retina displayed the same staining pattern, but with a stronger intensity especially for the ED1-staining (D–F). Graph shows numbers of microglial cells and data is given as mean ±SD (n = 4 explants/group). * p <0.05 compared to control. GCL = ganglion cell layer, INL = inner nuclear layer, IPL = inner plexiform layer, ONL = outer nuclear layer, OPL = outer plexiform layer. Scale bar equals 200 µm.

Article Snippet: Sections were then incubated with primary antibodies, rabbit anti-glial fibrillary acidic protein (GFAP, 1∶1500 DAKO Cytomation, Glostrup Denmark), rabbit anti- Iba1 (1∶200, WAKO, Japan), and rat anti-mouse ED1 (CD68, 1∶1000, Nordic Biosite, Sweden), 1∶1000, overnight at 4°C overnight, and thereafter detected by incubation in secondary antibodies for 2 h. Secondary antibodies included were Texas Red-conjugated donkey anti-rabbit antibody (1∶200; Abcam, Cambridge, UK), Alexa 488 goat anti-rabbit IgG (Molecular Probes) and Alexa 564 goat anti-rat (Molecular Probes).

Techniques: Immunostaining, Control, Staining

Journal: PLoS ONE

Article Title: Silver and Gold Nanoparticles Exposure to In Vitro Cultured Retina – Studies on Nanoparticle Internalization, Apoptosis, Oxidative Stress, Glial- and Microglial Activity

doi: 10.1371/journal.pone.0105359

Figure Lengend Snippet: A–D. Iba1-immunohistochemistry revealing microglial cells with ramified (A), intermediate (B), round (C) and amoeboid (D) morphology, respectively. Quantification of the fractions of microglia in three different stages of activation was performed: intermediate or “early activated” cells as judged from the morphology and co-expression of ED1 while round- and amoeboid morphologies are regarded as “late activated” cells. The graph shows the distribution of activation stages, normalized to total numbers of Iba1-positive cells/explant; values ±SD (n = 4 explants/group) are given. * p <0.05 compared to control. GCL = ganglion cell layer; INL = inner nuclear layer; ONL = outer nuclear layer. Scale bars equal 50 µm.

Article Snippet: Sections were then incubated with primary antibodies, rabbit anti-glial fibrillary acidic protein (GFAP, 1∶1500 DAKO Cytomation, Glostrup Denmark), rabbit anti- Iba1 (1∶200, WAKO, Japan), and rat anti-mouse ED1 (CD68, 1∶1000, Nordic Biosite, Sweden), 1∶1000, overnight at 4°C overnight, and thereafter detected by incubation in secondary antibodies for 2 h. Secondary antibodies included were Texas Red-conjugated donkey anti-rabbit antibody (1∶200; Abcam, Cambridge, UK), Alexa 488 goat anti-rabbit IgG (Molecular Probes) and Alexa 564 goat anti-rat (Molecular Probes).

Techniques: Immunohistochemistry, Activation Assay, Expressing, Control